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It is now well known that microorganisms present at the skin surface are in perfect symbiosis with the skin. We can even say now that skin is made of a human and a microbial part, this is the concept of the holobiont (2). Anyway microbes are the first range of protection against external aggression causing skin aging.

Metaproteomics is able to reach in an untargeted way both human and microbial proteins together, coupled with bioinformatics and AI data processing. Based on a relative quantification of proteins between conditions made by nanoLC-MS/MS, it allows to more precisely understand what are the mechanisms involved in effects.(3) (4)

Our publication with partners (1) shows by comparison of young and aged dehydrated skins and in protection or restoration by a powerful ingredient at the taxonomic and biological functional levels .

https://www.mdpi.com/2079-9284/11/4/132

As kinetics and level of expression of omics are different, the multi-omics tendancy is to couple different omics in order to paint a much more comprehensive and detailed picture of what is occurring between the host and microbes in healthy and diseased states(1).

Recently, new studies on skin used metagenomics coupled to metabolomics (2) (4). Of course metabolomics is attractive as being the final stage of the metabolization of substrates and reaching the molecular forms of exchanges within the microbiota and with the host. Even if limitations like only 10% of microbial and human metabolites being identified in the databases (5), it remains interesting to know more about for example fatty acids which are the most important components in maintaining the skin barrier function or lactic acid which is a key component of the skin’s natural moisturizing factor and keeps skin hydrated (4), or lipids as key components of the skin (3).

Then, it is logical to couple the omics which are in charge of the functions and which detect the production of the functions, namely metaproteomics and metabolomics(6), which will better track the host-microbes interactions (2), as metaproteomics reaches simultaneously the proteins of the skin and its microbiota which produce the metabolites to be detected. It is particularly efficient as it is often seen that there is no change at skin microbial abundance, taxonomic or functional metagenomics level but shifts appear at the real functional and metabolic levels , as discussed by these authors.(4)

PHYLOGENE is familiarin using the complementarity between metagenomics and metaproteomics to obtain more focused claims .

Now based on its expertise in metaproteomics, PHYLOGENE is completing its proposed services as close as possible to real functionality with metabolomics dedicated to skin metabolism . This metaproteomics and metabolomics toolset makes it possible to generate knowledge of the effects of skin diseases or the mechanisms of action of cosmetics, prebiotics, probiotics or drugs targeting the skin microbiome.

Metaproteomics:

Proteins are in charge of cellular structures and functions of life activities. Proteomics provides insights into the composition and activity patterns of intracellular proteins and protein-protein interactions.

Metaproteomics makes it possible to achieve simultaneously the identification of the microbiome members, and a snapshot of the functions of the host and its microbiomes. The non-targeted approach gives the identification and relative quantification of the host and its microbiome proteins impacted by the studied phenomenon and produces a set of data reflecting the studied effect.

Metabolomics :

Metabolites are the molecules produced by the functional proteome in response to environmental stimuli. Metabolomics is the final stage of the metabolization of substrates and identifies the molecular forms of exchanges within the microbiota and with the host. The proposed approach is a targeted approach of relative quantification metabolites known for their role in the skin metabolism.

Multiomics :

With coupled metaproteomics and metabolomics, we use the potential of the two omics closest to the phenotype.

Our bioinformatics tools enable integrated and standardized proteomic and metabolomic analysis of global proteome and metabolome data. Enrichment of metabolic pathways and correlation analysis of regulatory mechanisms of upstream protein actors of metabolism and downstream metabolite changes provide a comprehensive view of the effects generated at the gut level.

 (1) Simran S Sandhu, Aunna Pourang, Raja K Sivamani. A review of next generation sequencing technologies used in the evaluation of the skin microbiome: what a time to be alive. 2019 Dermatology on line journal  Volume 25 Number 7| July 2019| 25(7):1  

(2) Jacques C and all. Multi-omics approach to understand the impact of sun exposure on an in vitro skin ecosystem and evaluate a new broad-spectrum sunscreen.

Photochem Photobiol. 2023 Jul 24. ;00 :1-14

(3) Patra V and all. Ultraviolet exposure regulates skin metabolome based on the microbiome. Sci Rep. 2023; 13: 7207.

(4) Li M and all. Multi-omic approach to decipher the impact of skincare products with pre/postbiotics on skin microbiome and metabolome. Front Med (Lausanne). 2023; 10: 1165980.

(5) Peisl BYL and all. Dark matter in host-microbiome metabolomics: Tackling the unknowns–A review. Analytica Chimica Acta Volume 1037, 11 December 2018, Pages 13-27

(6) Oleg Karaduta, Zeljko Dvanajscak,and Boris Zybailov. Metaproteomics—An Advantageous Option in Studies of Host-Microbiota Interaction. Microorganisms. 2021 May; 9(5): 980.

Nous avons plusieurs projets qui arrivent en parallèle, nous renforçons notre équipe et recherchons un chef de projet. Venez nous rencontrer, nous ferons du chemin ensemble.

  PHYLOGENE to talk about omics technos for microbiome products effects substantiation

PHYLOGENE a été désigné meilleur poster à Targeting Microbiota 2022. Bravo à toute l'équipe

Recevez au laboratoire un kit de biopsies de peau de CTIBiotech, appliquez votre produit, envoyez les biopsies chez Phylogene pour l'étude de protéomique, recevez vos résultats.

Responsable spectrométrie de masse

Notre poster à SID 2022 avec des partenaires pour l'évaluation des effets d'un ingrédient sur la peau et son microbiote

DeepMicro: PHYLOGENE et le CEA s'associent dans un projet pour développer la métaprotéomique des microbiomes